FIG. 2. Separation and characterization of the
PSI-enriched fraction. a, gradient A is the PSI-enriched fraction
from normally grown Synechocystis cells, and Gradient
B is the PSI-enriched fraction from iron-stressed cells. b,
SDS-polyacrylamide gel electrophoresis and immunoblotting of
Gradient B fractions. M, molecular mass markers; lane 1, free
CP43'; lane 3 - PSI trimer; lane 4, CP43'- PSI complex; Blot,
immunoblotting using an antibody to CP43' kindly supplied by
Professor L. Sherman (Purdue University). The band marked with
an asterisk, seen both in Gradients A and B, is attributed to
free carotenoids, whereas Band 2 containing monomeric PSI gave
the same SDS-polyacrylamide gel electrophoresis profile as Band
3.
