Figure 5

Fig. 5. Detailed modelling of the projection map for the LHCI–PSI supercomplex isolated from C. reinhardtii cells placed in State 2. Modelling is based on higher plant coordinates 1qzv.pdb with PSI core (green), LHCI antenna (red), PsaJ (yellow), PsaK (magenta), PsaG (purple), PsaI (orange), PsaL (cyan) and PsaH (white). The additional density observed in State 2 LHCI–PSI supercomplex which is able to accommodate an additional LHC subunit is coloured blue and is suggested to be phospho-CP29 (see text). Chlorophylls are shown in yellow, but were excluded from the additional density attributed to the LHCI and CP29 subunits. The detergent shell surrounding the particles in the hydrophobic membrane plane sits within any stain present and this shell is assigned here as an ~15 A wide outer contour (yellow). Scale bar = 50 A.

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