Probing the organization of photosystem II in photosynthetic membranes by atomic force microscopy: Biochemistry in press.
Helmut Kirchhoff, Steven Lenhert, Claudia Büchel, Lifeng Chi & Jon Nield

Figure 3

Figure 3 Topographical images of a grana area (area in upper part of Figure 2B). (A) bilayer level (see text for definition), (B) protrusion level which is about 2 nm higher than the bilayer level. Clearly separated protrusions with a height of 4 to 6 nm are visible. (C) Result of a single particle analysis (566 particles) of the objects in the protrusion level (example see Figure 3B). (D) High resolution structure of the lumenal part of the PSII-dimer (.pdb file 1S5L). Only the part which protrudes ~ 4 nm from the bilayer level is shown, together with an overlay of the similar density resolved at 1.7 nm resolution from cryo-EM derived data. (E) Low resolution 2D projection deduced by mathematical filtering from the cyanobacterial PSII underlying co-ordinates of (D). The resolution of ~ 4 nm produced by the filtering was chosen as similar to the resolution of our AFM images. Scale bar in (D) is 10 nm.

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