Figure 1. Sucrose density gradient and characterization
of proteins in the green fractions. (a) Sucrose density gradient
showing separation of four clear fractions, F1–F4. (b)
Coomassie blue-stained SDS-PAGE of the thylakoid membrane and
fractions obtained by sucrose density gradient centrifugation.
(c) Immunoblotting with polyclonal Abs raised to PsaA PSI proteins
of Chlamydomonas and PsbA (D1) PSII protein of Synechocystis
6803. Lanes 1–4 are for fractions F1–F4 as identified
by sucrose density centrifugation. Th, thylakoids; M, molecular
mass markers (in kDa).
