FIG. 6. Phosphorylation status of C. reinhardtii
thylakoids used for purification of the LHCI-PSI supercomplex.
Molecular mass of protein markers (in kDa) is indicated on the
left. T, thylakoids; .T, DDM-solubilized thylakoids following
partial PSII depletion; F1- F3, sucrose gradient fractions as
described in Fig. 1 legend. Proteins were loaded on SDS-PAGE
at 2 .g of Chl. Immunodetection of phosphorylated proteins was
performed using anti-phosphothreonine antiserum (see "Materials
and Methods").
