
FIG. 2. Protein composition of sugradient fractions
obtained from solubilized C. reinhardtii thylakoids. a, SDS-PAGE
of sucrose gradient fractions. b, Western detection of PSI core
and LHCI subunits. M, prestained protein markers with their
apparent molecular mass (in kDa) indicated on the left; T, thylakoids;
.T, DDM-solubilized thylakoids following partial PSII depletion;
F1- see Fig. 1 legend. Proteins were loaded at 5 and 2 .g Chl
in a and b, respectively.